Pump-free SERS microfluidic chip based on an identification-competition strategy for ultrasensitive and efficient simultaneous detection of liver cancer-related microRNAs.

ABSTRACT: In this study, we present a pump-free SERS microfluidic chip capable of detecting liver cancer-related miR-21 and miR-155 concurrently with ultra-sensitivity and high efficiency. We employed a Fe₃O₄@cDNA-AuNPs@Raman reporter@H composite structure and a recognition competition strategy. When the target miRNAs (miR-21 and miR-155) are present in the test liquid, they specifically compete with the nucleic acid complementary strand(H) of Fe₃O₄@cDNA-AuNPs@Raman reporter@H, causing AuNPs to competitively detach from the surface of Fe₃O₄, resulting in a decrease in the SERS signal. Consequently, this pump-free SERS microfluidic chip enables the detection of the target miRNAs more rapidly and accurately in complex environments. This

SUBMITTER: Zhou R 

PROVIDER: S-EPMC11563321 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature