Isobaric crosslinking mass spectrometry technology for studying conformational and structural changes in proteins and complexes.

ABSTRACT: Dynamic conformational and structural changes in proteins and protein complexes play a central and ubiquitous role in the regulation of protein function, yet it is very challenging to study these changes, especially for large protein complexes, under physiological conditions. Here, we introduce a novel isobaric crosslinker, Qlinker, for studying conformational and structural changes in proteins and protein complexes using quantitative crosslinking mass spectrometry. Qlinkers are small and simple, amine-reactive molecules with an optimal extended distance of ~10 Å, which use MS2 reporter ions for relative quantification of Qlinker-modified peptides derived from different samples. We synthesized the 2-plex Q2linker and showed that the Q2linker can provide quantitative crosslinking data that

SUBMITTER: Luo J 

PROVIDER: S-EPMC11563578 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature