The impact of isoflurane anesthesia on brain metabolism in mice: An MRI and electroencephalography study.

ABSTRACT: Isoflurane is one of the most widely used anesthetic agents in rodent imaging studies. However, the impact of isoflurane on brain metabolism has not been fully characterized to date, primarily due to a scarcity of noninvasive technologies to quantitatively measure the brain's metabolic rate in vivo. In this study, using noncontrast MRI techniques, we dynamically measured cerebral metabolic rate of oxygen (CMRO₂) under varying doses of isoflurane anesthesia in mice. Concurrently, systemic parameters of heart and respiration rates were recorded alongside CMRO₂. Additionally, electroencephalogram (EEG) recording was used to identify changes in neuronal activities under the same anesthetic regimen employed in the MRI experiments. We found suppression of the CMRO₂

SUBMITTER: Wei Z 

PROVIDER: S-EPMC11563868 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature