Structure of Essential RNA Regulatory Elements in the West Nile Virus 3'-Terminal Stem Loop.

ABSTRACT: Flaviviruses, such as West Nile and Dengue Virus, pose a significant and growing threat to global health. Central to the flavivirus life cycle are highly structured 5'- and 3'-untranslated regions (UTRs), which harbor conserved cis-acting RNA elements critical for viral replication and host adaptation. Despite their essential roles, detailed molecular insights into these RNA elements have been limited. By employing nuclear magnetic resonance (NMR) spectroscopy in conjunction with SAXS experiments, we determined the three-dimensional structure of the West Nile Virus (WNV) 3'-terminal stem-loop core, a highly conserved element critical for viral genome cyclization and replication. Single nucleotide mutations at several sites within this RNA abolish the ability of the virus to replicate. Thes

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC11563921 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature