A multi-modal single-cell and spatial expression map of metastatic breast cancer biopsies across clinicopathological features.

ABSTRACT: Although metastatic disease is the leading cause of cancer-related deaths, its tumor microenvironment remains poorly characterized due to technical and biospecimen limitations. In this study, we assembled a multi-modal spatial and cellular map of 67 tumor biopsies from 60 patients with metastatic breast cancer across diverse clinicopathological features and nine anatomic sites with detailed clinical annotations. We combined single-cell or single-nucleus RNA sequencing for all biopsies with a panel of four spatial expression assays (Slide-seq, MERFISH, ExSeq and CODEX) and H&E staining of consecutive serial sections from up to 15 of these biopsies. We leveraged the coupled measurements to provide reference points for the utility and integration of different experimental techniques and used

SUBMITTER: Klughammer J 

PROVIDER: S-EPMC11564109 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature