Advances in methods and concepts provide new insight into antibiotic fluxes across the bacterial membrane.

ABSTRACT: The sophisticated envelope of Gram-negative bacteria modulates the uptake of small molecules in a side-chain-sensitive manner. Despite intensive theoretical and experimental investigations, a general set of pathways underpinning antibiotic uptake has not been identified. This manuscript discusses the passive influx versus active efflux of antibiotics, considering the responsible membrane proteins and the transported molecules. Recent methods have analyzed drug transport across the bacterial membrane in order to understand their activity. The combination of in vitro, in cellulo and in silico methods shed light on the key, mainly electrostatic, interactions between the molecule surface, porins and transporters during permeation. A key factor is the relationship between the dose of an active

SUBMITTER: Vergalli J 

PROVIDER: S-EPMC11564671 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature