LINC00261 triggers DNA damage via the miR-23a-3p/CELF2 axis to mitigate the malignant characteristics of ¹³¹I-resistant papillary thyroid carcinoma cells.

ABSTRACT:

Background

Long-chain non-coding RNA (LINC00261) in the treatment of papillary thyroid carcinoma (PTC) with ¹³¹I is still unknown despite its proven anti-tumour effect in thyroid cancer (TC) and other types of cancer.

Methods

The database and RT-qPCR were used to analyze the expression level of LINC00261 in PTC and cell lines. PTC cells resistant to ¹³¹I (TPC-1/R) were created through ongoing exposure to a lethal dose of ¹³¹I, and a subcutaneous xenotransplantation model was developed using PTC mice. Bioinformatics analysis and dual-luciferase assays demonstrated the interaction between LINC00261, miR-23a-3p, and CELF2. RT-qPCR and Western blot were used to detect the expression of LINC00261, miR-23a-3p, and CELF2. Additionally, CCK-8, flow c

SUBMITTER: Tao Q 

PROVIDER: S-EPMC11564912 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature