Sulforaphane attenuates phosgene-induced acute lung injury via the Nrf2-HO-1/NQO1 pathway.

ABSTRACT:

Background

Sulforaphane (SFN) has been demonstrated to exert a protective role in various diseases. However, the role of SFN in phosgene-induced acute lung injury (P-ALI) remains unclear. This study aimed to explore the role and mechanism of SFN in P-ALI and provide a theoretical basis for the clinical prevention and treatment of P-ALI.

Methods

A mouse model of P-ALI was established followed by phosgene gas inhalation at a dose of 4.17 g/m³ for 5 min. The survival rate, lung coefficient and hematoxylin and eosin (H&E) staining, lung pathology scoring, and bronchoalveolar lavage fluid (BALF) analysis were performed to evaluate lung tissue damage. The real-time quantitative polymerase chain reaction (RT-qPCR) and Western blotting analysis were utilized to evaluate t

SUBMITTER: Lu Q 

PROVIDER: S-EPMC11565325 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature