Imbalance of intestinal flora activates inflammatory response contributing to acute lung injury.

ABSTRACT:

Background

Seawater drowning is a leading cause of accidental injury and death, and the resulting acute lung injury (ALI) is a serious clinical syndrome for which there are no effective treatments. This study aims to investigate the potential mechanism of seawater drowning-induced ALI.

Methods

Seawater drowning mouse models were constructed to assess lung injury. The hematoxylin & eosin (H&E) staining and fluorescent terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay were used to observe the pathology of lung tissues and apoptosis, respectively. 16S rRNA and RNA-seq were performed to identify the structure of the intestinal microbes and the gene expression profiles of the lung tissue of the mice, respectively. The expression of cytokines was

SUBMITTER: He Y 

PROVIDER: S-EPMC11565338 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature