Mechanistic insights into structure-based design of a Lyme disease subunit vaccine.

ABSTRACT: The quality of protective immunity plays a critical role in modulating vaccine efficacy, with native antigens often not able to trigger sufficiently strong immune responses for pathogen killing. This warrants creation of structure-based vaccine design, leveraging high-resolution antigen structures for mutagenesis to improve protein stability and efficient immunization strategies. Here, we investigated the mechanisms underlying structure-based vaccine design using CspZ-YA, a vaccine antigen from Borrelia burgdorferi, the bacteria causing Lyme disease (LD), the most common vector-borne disease in the Northern Hemisphere. Compared to wild-type CspZ-YA, we found CspZ-YA_I183Y and CspZ-YA_C187S required lower immunization frequency to protect mice from LD-associated m

SUBMITTER: Brangulis K 

PROVIDER: S-EPMC11565809 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature