Histone variant H2BE controls activity-dependent gene expression and homeostatic scaling.

ABSTRACT: A cell's ability to respond and adapt to environmental stimuli relies in part on transcriptional programs controlled by histone proteins. Histones affect transcription through numerous mechanisms including through replacement with variant forms that carry out specific functions. We recently identified the first widely expressed H2B histone variant, H2BE and found that it promotes transcription and is critical for neuronal function and long-term memory. However, how H2BE is regulated by extracellular stimuli and whether it controls activity-dependent transcription and cellular plasticity remain unknown. We used CUT&Tag and RNA-sequencing of primary neurons, single-nucleus sequencing of cortical tissue, and multielectrode array recordings to interrogate the expression of H2BE in response to

SUBMITTER: Feierman ER 

PROVIDER: S-EPMC11565944 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature