<i>N</i>-Aldehyde-Modified Phosphatidylethanolamines generated by lipid peroxidation are robust substrates of <i>N</i>-Acyl Phosphatidylethanolamine Phospholipase D.

ABSTRACT: N-acyl phosphatidylethanolamine-hydrolyzing phospholipase D (NAPE-PLD) hydrolyzes phosphatidylethanolamines (PE) where the headgroup nitrogen has been enzymatically modified with acyl chains of four carbons or longer (N-acyl-PEs or NAPEs). The nitrogen headgroup of PE can also be non-enzymatically modified by reactive lipid aldehydes, thus forming N-aldehyde modified-PEs (NALPEs). Some NALPEs such as N-carboxyacyl-PEs are linked to PE via amide bonds similar to NAPEs, but others are linked by imine, pyrrole, or lactam moieties. Whether NAPE-PLD can hydrolyze NALPEs was unknown. We therefore characterized the major NALPE species formed during lipid peroxidation of arachidonic acid and linoleic acid and generated various NALPEs for characterization of their sensit

SUBMITTER: Fadaei R 

PROVIDER: S-EPMC11565945 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature