High-throughput Generation of Collagen Microbeads for 3D Cell Culture and Extracellular Vesicle Production.

ABSTRACT: Collagen type I, a fundamental component of natural extracellular matrices across species, is an attractive material for the development of tissue engineering constructs. Nevertheless, the poor mechanical properties and thermal instability hinder its use for specific construction of 3D cultures and 3D printing. In this study, we present a microfluidic high-throughput approach for producing high-quality and uniform collagen microbeads without introducing any chemical modification. We achieved rapid and uniform collagen droplet fabrication with sizes spanning from 50 μm to 1200 μm in a production rate of up to 10000 droplets per minute. The resulting collagen microbeads can serve as numerous microbioreactors which are suspended in the culture medium without precipitation and are ideal for 3D

SUBMITTER: Ali S 

PROVIDER: S-EPMC11566015 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature