An optimized Langendorff-free method for isolation and characterization of primary adult cardiomyocytes.

ABSTRACT: Isolation of adult mouse cardiomyocytes is an essential technique for advancing our understanding of cardiac physiology and pathology, and for developing therapeutic strategies to improve cardiac health. Traditionally, cardiomyocytes are isolated from adult mouse hearts using the Langendorff perfusion method in which the heart is excised, cannulated, and retrogradely perfused through the aorta. While this method is highly effective for isolating cardiomyocytes, it requires specialized equipment and technical expertise. To address the challenges of the Langendorff perfusion method, researchers have developed a Langendorff-free technique for isolating cardiomyocytes. This Langendorff-free technique involves anterograde perfusion through the coronary vasculature by clamping the aorta and intr

SUBMITTER: Nikouee A 

PROVIDER: S-EPMC11566262 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature