A rotenone organotypic whole hemisphere slice model of mitochondrial abnormalities in the neonatal brain.

ABSTRACT: Mitochondrial abnormalities underscore a variety of neurologic injuries and diseases and are well-studied in adult populations. Clinical studies identify critical roles of mitochondria in a wide range of developmental brain injuries, but models that capture mitochondrial abnormalities in systems representative of the neonatal brain environment are lacking. Here, we develop an organotypic whole-hemisphere (OWH) brain slice model of mitochondrial dysfunction in the neonatal brain. We extended the utility of complex I inhibitor rotenone (ROT), canonically used in models of adult neurodegenerative diseases, to inflict mitochondrial damage in OWH slices from term-equivalent rats. We quantified whole-slice health over 6 days of exposure for a range of doses represented in ROT literature. We iden

SUBMITTER: Butler B 

PROVIDER: S-EPMC11566268 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature