The metabolic engineering of Escherichia coli for the high-yield production of hypoxanthine.

ABSTRACT:

Background

Hypoxanthine, prevalent in animals and plants, is used in the production of food additives, nucleoside antiviral drugs, and disease diagnosis. Current biological fermentation methods synthesize quantities insufficient to meet industrial demands. Therefore, this study aimed to develop a strain capable of industrial-scale production of hypoxanthine.

Results

De novo synthesis of hypoxanthine was achieved by blocking the hypoxanthine decomposition pathway, thus alleviating transcriptional repression and multiple feedback inhibition, and introducing a purine operon from Bacillus subtilis to construct a chassis strain. The effects of knocking out the IMP(Inosine 5'-monophosphate) branch on the growth status and titer of the strain were then investigated, and the effecti

SUBMITTER: Zhao S 

PROVIDER: S-EPMC11566304 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature