Unveiling the chemotherapeutic potential of two platinum(IV) complexes in skin cancer: <i>in vitro and in vivo</i> Insights.

ABSTRACT: The present study investigates the chemotherapeutic potential of two platinum (IV) complexes, P-PENT and P-HEX, against skin cancer in vitro and in vivo. Both complexes exhibited potent cytotoxicity against HaCaT-II-4 cells with IC₅₀ values of 0.8 ± 0.08 μM and 1.3 ± 0.16 μM respectively, while demonstrating 8-10-fold selectivity compared to mesenchymal stem cells (MSCs). Western blot analysis revealed significant modulation of key apoptotic and survival pathways, including upregulation of Bax/Bcl2 ratio, cleaved caspase 3, and cytochrome c, suggesting induction of intrinsic apoptosis. The complexes also inhibited PI3K and MAPK pathways, as evidenced by decreased p-AKT/AKT and p-ERK/ERK ratios. Flow cytometry confirmed significant apoptotic cell death. Both complex

SUBMITTER: Slika A 

PROVIDER: S-EPMC11566320 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature