A green deep eutectic solvent-based aqueous two-phase system combined with chemometrics for flavonoids extracting and detecting in honey.

ABSTRACT: In this study, an eco-friendly and designable aqueous two-phase system (ATPS) was developed using natural deep eutectic solvents (DES) and short-chain alcohols. The formation mechanism and influence of various factors on phase behavior were investigated. Optimal extraction parameters were determined through single-factor experiments and response surface methodology (RSM): 45 °C temperature, 45 % n-propanol, 40 % DES, and 0.06 mL quercetin working standard solution. Based on the alternating trilinear decomposition assisted multiple curve resolution (ATLD-MCR) algorithm, a calibration model was established for simultaneous and rapid quantitative analysis of flavonoids in Acacia honey, achieving 74.0-86.6 % accuracy and 0.82-2.20 % standard deviation. Moreover, the green chemistry metr

SUBMITTER: Zhang XH 

PROVIDER: S-EPMC11566322 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature