Mapping multi-regional functional connectivity of astrocyte-neuronal networks during behaviors.

ABSTRACT:

Significance

Diverse behaviors rely on coordinated activity and multi-regional functional connectivity within astrocyte-neuronal networks. However, current techniques for simultaneously measuring astrocytic and neuronal activities across multiple brain regions during behaviors remain limited.

Aim

We propose a multi-fiber solution that can simultaneously record activities of astrocyte-neuronal networks across multiple regions during behaviors.

Approach

We employed cell-specific dual-color genetically encoded calcium indicators (GECIs) and multi-fiber photometry to simultaneously measure astrocytic and neuronal Ca²⁺ transients across multiple brain regions in freely behaving animals.

Results

Our findings demonstrate that both movements and sensory stim

SUBMITTER: Wang H 

PROVIDER: S-EPMC11566604 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature