False Discovery Rate Estimation for Hybrid Mass Spectral Library Search Identifications in Bottom-up Proteomics.

ABSTRACT: We present a method for FDR estimation of mass spectral library search identifications made by a recently developed method for peptide identification, the hybrid search, based on an extension of the target-decoy approach. In addition to estimating confidence for a given identification, this allows users to compare and integrate identifications from the hybrid mass spectral library search method with other peptide identification methods, such as a sequence database-based method. In addition to a score, each hybrid score is associated with a "DeltaMass" value, which is the difference in mass of the search and library peptide, which can correspond to the mass of a modification. We explored the relation between FDR and DeltaMass using 100 concatenated random decoy libraries and discovered that

SUBMITTER: Burke MC 

PROVIDER: S-EPMC11566722 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature