Potential consequences of phototoxicity on cell function during live imaging of intestinal organoids.

ABSTRACT: Live imaging visualizes the structure, dynamics, and function of cells and tissues to reveal the molecular mechanisms, and has contributed to the advancement of life science. In live imaging, it has been well known that there is a trade-off between higher-resolution analysis and cell damage caused by light illumination, i.e., phototoxicity. However, despite the risk of unknowingly distorting experimental results, phototoxicity is an unresolved issue in live imaging because overall consequences occurring inside cells due to phototoxicity remains unknown. Here, we determined the molecular process of phototoxicity-induced cell damage systematically under low- and high-dose light illumination conditions by analyzing differential gene expression using RNA-sequencing in a three-dimensional organ

SUBMITTER: Yokoi Y 

PROVIDER: S-EPMC11567556 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature