Comprehensive mapping of somatotroph pituitary neuroendocrine tumour heterogeneity using spatial and single-cell transcriptomics.

ABSTRACT:

Background

Pituitary neuroendocrine tumours (PitNETs) are common intracranial tumours that are highly heterogeneous with unpredictable growth patterns. The driver genes and mechanisms that are crucial for tumour progression in somatotroph PitNETs are poorly understood.

Methods

In this study, we performed integrative spatial transcriptomics (ST) and single-cell RNA sequencing (scRNA-seq) analysis on somatotroph tumours and normal pituitary samples to comprehensively characterize the differences in cellular characteristics.

Results

By analyzing combined copy number variations (CNVs), tumour tissues were divided into two regions, which included the CNV_high and CNV_low areas. The protumour genes DLK1 and RCN1 were highly expressed in the CNV_high<

SUBMITTER: Wang J 

PROVIDER: S-EPMC11567828 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature