GRK2 mediates cisplatin-induced acute liver injury via the modulation of NOX4.

ABSTRACT:

Background

The present study investigated the function of G protein-coupled receptor kinase 2 (GRK2) in acute liver injury (ALI) by cisplatin, and investigated the protective effect of pharmacological inhibition of GRK2.

Methods

ALI models were generated in global adult hemizygous (ALI-Grk2^±) mice and wild-type (WT) mice. Liver biochemistry parameters and histopathology were used to evaluate the severity of ALI and the protective effect of pharmacological inhibition of GRK2. GRK2-siRNA was used to knock down the expression of GRK2 in AML12 cells in vitro.

Results

ALI model mice exhibited increased blood levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels and abnormal liver pathology accompanied by imbalanced L-glutathione (G

SUBMITTER: Wang Q 

PROVIDER: S-EPMC11567994 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature