Combined oxygen and glucose oscillations distinctly change the transcriptional and physiological state of Escherichia coli.

ABSTRACT: Escherichia coli, a common microbial host for industrial bioproduction, experiences a highly dynamic environment in industrial-scale bioreactors due to significant glucose and oxygen gradients. In this study, we mimic the combined gradients of glucose and oxygen in high-throughput bioreactors to study the transcriptional response of E. coli to industrial-scale conditions. Under oscillating oxygen conditions, E. coli formed less biomass and accumulated the anaerobic by-product acetate. With respect to oxygen-responsive genes, we found that genes of the TCA cycle and of different electron transport chain complexes were differentially expressed. A global analysis of the expression data revealed that oxygen oscillations had caused a transition towards a catabolite-repressed state and upregulat

SUBMITTER: Bafna-Ruhrer J 

PROVIDER: S-EPMC11568247 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature