Piezo activity levels need to be tightly regulated to maintain normal morphology and function in pericardial nephrocytes.

ABSTRACT: Due to their position on glomerular capillaries, podocytes are continuously counteracting biomechanical filtration forces. Most therapeutic interventions known to generally slow or prevent the progression of chronic kidney disease appear to lower these biomechanical forces on podocytes, highlighting the critical need to better understand podocyte mechano-signalling pathways. Here we investigated whether the mechanotransducer Piezo is involved in a mechanosensation pathway in Drosophila nephrocytes, the podocyte homologue in the fly. Loss of function analysis in Piezo depleted nephrocytes reveal a severe morphological and functional phenotype. Further, pharmacological activation of endogenous Piezo with Yoda1 causes a significant increase of intracellular Ca⁺⁺ upon exposure to a

SUBMITTER: Schulz K 

PROVIDER: S-EPMC11568303 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature