Analysis of immunogenic cell death in periodontitis based on scRNA-seq and bulk RNA-seq data.

ABSTRACT:

Background

Recent studies have suggested that cell death may be involved in bone loss or the resolution of inflammation in periodontitis. Immunogenic cell death (ICD), a recently identified cell death pathway, may be involved in the development of this disease.

Methods

By analyzing single-cell RNA sequencing (scRNA-seq) for periodontitis and scoring gene set activity, we identified cell populations associated with ICD, which were further verified by qPCR, enzyme linked immunosorbent assay (ELISA) and immunofluorescence (IF) staining. By combining the bulk transcriptome and applying machine learning methods, we identified several potential ICD-related hub genes, which were then used to build diagnostic models. Subsequently, consensus clustering analysis was performed to ident

SUBMITTER: Wu E 

PROVIDER: S-EPMC11568468 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature