Fungal-specific PCR primers developed for analysis of the ITS region of environmental DNA extracts.

ABSTRACT:

Background

The Internal Transcribed Spacer (ITS) regions of fungal ribosomal DNA (rDNA) are highly variable sequences of great importance in distinguishing fungal species by PCR analysis. Previously published PCR primers available for amplifying these sequences from environmental samples provide varying degrees of success at discriminating against plant DNA while maintaining a broad range of compatibility. Typically, it has been necessary to use multiple primer sets to accommodate the range of fungi under study, potentially creating artificial distinctions for fungal sequences that amplify with more than one primer set.

Results

Numerous sequences for PCR primers were tested to develop PCR assays with a wide range of fungal compatibility and high discrimination from plant DNA

SUBMITTER: Martin KJ 

PROVIDER: S-EPMC1156903 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature