Full-length RNA-Seq of the RHOH gene in human B cells reveals new exons and splicing patterns.

ABSTRACT: The RhoH protein is a member of the Ras superfamily of guanosine triphosphate-binding proteins. RhoH is an atypical Rho family member that is always GTP-bound and thus always activated. It is restrictively expressed in normal hematopoietic cells, where it is a negative regulator of cell growth and survival. We previously analyzed the RHOH gene structure and demonstrated that this gene is composed of 7 exons, one single encoding exon located at the 3' extremity of the gene, preceded by 6 noncoding exons. To further understand the transcription events associated with this gene, we performed full-length RNA-Seq on 12 B-cell lines. We identified new exons, new splice events and new splice sites, leading to the discovery of 38 RHOH mRNA molecules, 27 of which have never been described before. H

SUBMITTER: Lepretre F 

PROVIDER: S-EPMC11569159 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature