Comparison of retinol binding protein 1 with cone specific G-protein as putative effector molecules in cryptochrome signalling.

ABSTRACT: Vision and magnetoreception in navigating songbirds are strongly connected as recent findings link a light dependent radical-pair mechanism in cryptochrome proteins to signalling pathways in cone photoreceptor cells. A previous yeast-two-hybrid screening approach identified six putative candidate proteins showing binding to cryptochrome type 4a. So far, only the interaction of the cone specific G-protein transducin α-subunit was investigated in more detail. In the present study, we compare the binding features of the G-protein α-subunit with those of another candidate from the yeast-two-hybrid screen, cellular retinol binding protein. Purified recombinant European robin retinol binding protein bound retinol with high affinity, displaying an EC₅₀ of less than 5 nM, thereby demons

SUBMITTER: Yee C 

PROVIDER: S-EPMC11569197 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature