Chrysalis: decoding tissue compartments in spatial transcriptomics with archetypal analysis.

ABSTRACT: Dissecting tissue compartments in spatial transcriptomics (ST) remains challenging due to limited spatial resolution and dependence on single-cell reference data. We present Chrysalis, a computational method that rapidly uncovers tissue compartments through spatially variable gene (SVG) detection and archetypal analysis without requiring external reference data. Additionally, it offers a unique visualisation approach for swift tissue characterisation and provides access to the underlying gene expression signatures, enabling the identification of spatially and functionally distinct cellular niches. Chrysalis was evaluated through various benchmarks and validated against deconvolution, independently obtained cell type abundance data, and histopathological annotations, demonstrating superior

SUBMITTER: Turos D 

PROVIDER: S-EPMC11569261 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature