Quantification of HTLV-I proviral load in experimentally infected rabbits.

ABSTRACT:

Background

Levels of proviral load in HTLV-1 infected patients correlate with clinical outcome and are reasonably prognostic. Adaptation of proviral load measurement techniques is examined here for use in an experimental rabbit model of HTLV-1 infection. Initial efforts sought to correlate proviral load with route and dose of inoculation and with clinical outcome in this model. These methods contribute to our continuing goal of using the model to test treatments that alleviate virus infection.

Results

A real-time PCR assay was used to measure proviral load in blood and tissue samples from a series of rabbits infected using HTLV-1 inocula prepared as either cell-free virus particles, infected cells or blood, or by naked DNA injection. Proviral loads from asymptomatically infe

SUBMITTER: Zhao TM 

PROVIDER: S-EPMC1156951 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature