Expression of Chondrogenic Potential Markers in Cultured Chondrocytes from the Human Knee Joint.

ABSTRACT: ObjectivesWhile substantial progress has been made in engineering cartilaginous constructs for animal models, further research is needed to translate these methodologies for human applications. Evidence suggests that cultured autologous chondrocytes undergo changes in phenotype and gene expression, thereby affecting their proliferation and differentiation capacity. This study was designed to evaluate the expression of chondrogenic markers in cultured human articular chondrocytes from passages 3 (P3) and 7 (P7), beyond the current clinical recommendation of P3.MethodsCultured autologous chondrocytes were passaged from P3 up to P7, and quantitative polymerase chain reaction (qPCR) was used to assess mRNA expression of chondrogenic markers, including collagen type I (COLI), collagen type II (

SUBMITTER: Bonello JP 

PROVIDER: S-EPMC11569588 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature