Engineering of Saccharomyces cerevisiae as a platform strain for microbial production of sphingosine-1-phosphate.

ABSTRACT:

Background

Sphingosine-1-phosphate (S1P) is a multifunctional sphingolipid that has been implicated in regulating cellular activities in mammalian cells. Due to its therapeutic potential, there is a growing interest in developing efficient methods for S1P production. To date, the production of S1P has been achieved through chemical synthesis or blood extraction, but these processes have limitations such as complexity and cost. In this study, we generated an S1P-producing Saccharomyces cerevisiae strain by using metabolic engineering and introducing a heterologous sphingolipid biosynthetic pathway to demonstrate the possibility of microbial S1P production.

Results

To construct the sphingosine-producing S. cerevisiae strain, both the sphingolipid delta 4 desaturase gene (DES1)

SUBMITTER: Jang IS 

PROVIDER: S-EPMC11569612 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature