Cellular Enhancement of Frozen Meniscus Allograft Combining Native Meniscus and Mesenchymal Stromal Cell Injections.

ABSTRACT: ObjectiveThis proof-of-concept study investigated an improved cell-based injection therapy combining mesenchymal stem cells (MSCs) and meniscus cells (MCs) to support superior meniscus allograft repopulation and early revival compared to injecting MSCs alone.DesignIn this controlled laboratory study, frozen meniscus allograft samples were injected vertically with a cell suspension containing different ratios of MSCs and MCs or control (lactated ringers) and cultured for 28 days. Samples were analyzed weekly for cell viability, migration, and metabolism using histological and biochemical assays. Tissue medium was analyzed for matrix metalloproteinase (MMP) expression using zymography.ResultsCellular repopulation of frozen allografts injected with different cell suspensions was validated by

SUBMITTER: Struijk C 

PROVIDER: S-EPMC11569627 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature