Tight junction protein changes in irritable bowel syndrome: the relation of age and disease severity.

ABSTRACT:

Background/aims

The etiology of irritable bowel syndrome (IBS) is associated with intestinal mucosal barrier damage. However, changes in the tight junction (TJ) proteins in IBS have not been fully elucidated. This study aimed to evaluate TJ protein changes in IBS patients and the relationship between aging and disease severity.

Methods

Thirty-six patients with IBS fulfilling the Rome IV criteria and twenty-four controls were included. To evaluate the change of TJ in the colonic mucosa, quantitative reverse transcription polymerase chain reaction, western blot, and immunohistochemistry (IHC) were performed, respectively.

Results

The entire IBS group (n = 36) exhibited decreased levels of claudin-1 and -2 mRNA compared to the control group (n = 24), with statistical sig

SUBMITTER: Kim SU 

PROVIDER: S-EPMC11569931 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature