Chromosome-scale genome assembly of Codonopsis pilosula and comparative genomic analyses shed light on its genome evolution.

ABSTRACT:

Introduction

Codonopsis pilosula is a significant plant in traditional Chinese medicine, valued for its edible and medicinal properties. However, the lack of available genomic resources has hindered further research.

Methods

This study presents the first chromosome-scale genome assembly of C. pilosula using PacBio CLR reads and Hi-C scaffolding technology. Additionally, Ks analysis and syntenic depth analysis were performed to elucidate its evolutionary history.

Results

The final assembly yielded a high-quality genome of 679.20 Mb, which was anchored to 8 pseudo-chromosomes with an anchoring rate of 96.5% and a scaffold N50 of 80.50 Mb. The genome assembly showed a high completeness of 97.6% based on Benchmarking with Universal Single-Copy Orthologs (BUS

SUBMITTER: Chen BZ 

PROVIDER: S-EPMC11570261 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature