A promiscuous mechanism to phase separate eukaryotic carbon fixation in the green lineage.

ABSTRACT: CO₂ fixation is commonly limited by inefficiency of the CO₂-fixing enzyme Rubisco. Eukaryotic algae concentrate and fix CO₂ in phase-separated condensates called pyrenoids, which complete up to one-third of global CO₂ fixation. Condensation of Rubisco in pyrenoids is dependent on interaction with disordered linker proteins that show little conservation between species. We developed a sequence-independent bioinformatic pipeline to identify linker proteins in green algae. We report the linker from Chlorella and demonstrate that it binds a conserved site on the Rubisco large subunit. We show that the Chlorella linker phase separates Chlamydomonas Rubisco and that despite their separation by ~800 million years of evolution, the Chlorella linker can s

SUBMITTER: Barrett J 

PROVIDER: S-EPMC11570498 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature