Preclinical evaluation of a universal inactivated influenza B vaccine based on the mosaic hemagglutinin-approach.

ABSTRACT: We have developed a new universal influenza B vaccination strategy based on inactivated influenza B viruses displaying mosaic hemagglutinins (mHAs). Recombinant mHA viruses were constructed by replacing the four major antigenic sites of influenza B virus HAs, with those from exotic avian influenza A virus HAs. Sequential vaccination of naïve mice with mHA-based vaccines elicited higher immune responses towards the immuno-subdominant conserved epitopes of the HA than vaccination with wildtype viruses. Among the different preparations tested, mHA split vaccines were less immunogenic than their whole inactivated virus counterparts. This lower immunogenicity was overcome by the combination with adjuvants. mHA split vaccines adjuvanted with a Toll-like receptor-9 agonist (CpG 1018) increased Th

SUBMITTER: Gonzalez-Dominguez I 

PROVIDER: S-EPMC11570629 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature