Influence of native thin filament type on the regulation of atrial and ventricular myosin motor activity.

ABSTRACT: Ca²⁺-mediated activation of thin filaments is a crucial step in initiating striated muscle contraction. To gain mechanistic insight into this regulatory process, thin filament (TF) components and myosin motors from diverse species and tissue sources are often combined in minimal in vitro systems. The contribution of tissue-specific TF composition with native myosin motors in generating contraction speed remains unclear. To examine TF-mediated regulation, we established a procedure to purify native TFs (nTF) and myosin motors (M-II) from the same cardiac tissue samples as low as 10 mg and investigated their influence on gliding speeds and Ca²⁺ sensitivity. The rabbit atrial and ventricular nTFs and M-II were assessed in in vitro nTF motility experiments under varying C

SUBMITTER: Spahiu E 

PROVIDER: S-EPMC11570844 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature