An intracellular phosphate buffer filters transient fluctuations in extracellular phosphate levels.

ABSTRACT: To survive in a dynamic and unpredictable environment, cells must correctly interpret and integrate extracellular signals with internal factors. In particular, internal stores of nutrients must be managed for use during periods of nutrient limitation. To gain insight into this complex process, we combined biochemical and spectroscopic techniques to follow the dynamics of the phosphate responsive signaling pathway in both single yeast cells and populations. We demonstrate that the phosphate-responsive genes PHO5 and PHO84 exhibit different kinetics of transcriptional induction in response to phosphate starvation, and that transient phosphate limitation causes induction of PHO84 but not PHO5. This differential kinetic behavior is largely eliminated in cells that lack the ability to store pho

SUBMITTER: Thomas MR 

PROVIDER: S-EPMC1157094 | biostudies-literature | 2005 Jul

REPOSITORIES: biostudies-literature