Insight into RecA-mediated repair of double strand breaks is provided by probing how contiguous heterology affects recombination.

ABSTRACT: Homologous recombination can promote correct repair of double strand breaks in DNA by aligning a sequence region in the broken chromosome with the corresponding sequence region in an unbroken chromosome. D-loops join the broken and unbroken chromosomes during homology testing. Previous work studied how some mismatches affect the stability of D-loops, but they did not probe whether the D-loops disrupt regions of contiguous mismatches or simply bypass them. Furthermore, previous work has not considered how the length of flanking homology affects D-loop disruption of regions of contiguous mismatches. Finally, there are conflicts about the polarity of D-loop extension. We demonstrate that with or without ATP hydrolysis invading strands with six contiguous mismatches and sufficient flanking hom

SUBMITTER: Danilowicz C 

PROVIDER: S-EPMC11570958 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature