Structure-based clustering and mutagenesis of bacterial tannases reveals the importance and diversity of active site-capping domains.

ABSTRACT: Tannins are critical plant defense metabolites, enriched in bark and leaves, that protect against microorganisms and insects by binding to and precipitating proteins. Hydrolyzable tannins contain ester bonds which can be cleaved by tannases-serine hydrolases containing so-called "cap" domains covering their active sites. However, comprehensive insights into the biochemical properties and structural diversity of tannases are limited, especially regarding their cap domains. We here present a code pipeline for structure prediction-based hierarchical clustering to categorize the whole family of bacterial tannases, and have used it to discover new types of cap domains and other structural insertions among these enzymes. Subsequently, we used two recently identified tannases from the gut/soil ba

SUBMITTER: Coleman T 

PROVIDER: S-EPMC11571031 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature