In vitro electrophysiological drug testing on neuronal networks derived from human induced pluripotent stem cells.

ABSTRACT:

Background

In vitro models for drug testing constitute a valuable and simplified in-vivo-like assay to better comprehend the biological drugs effect. In particular, the combination of neuronal cultures with Micro-Electrode Arrays (MEAs) constitutes a reliable system to investigate the effect of drugs aimed at manipulating the neural activity and causing controlled changes in the electrophysiology. While chemical modulation in rodents' models has been extensively studied in the literature, electrophysiological variations caused by chemical modulation on neuronal networks derived from human induced pluripotent stem cells (hiPSCs) still lack a thorough characterization.

Methods

In this work, we created three different configurations of hiPSCs-derived neuronal networks composed

SUBMITTER: Parodi G 

PROVIDER: S-EPMC11571758 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature