Functional interleukin-4 releasing microparticles impact THP-1 differentiated macrophage phenotype.

ABSTRACT:

Introduction

Macrophage cell therapies offer potential treatment in inflammatory diseases due to their ability to mobilize and stimulate their environment. However, successful treatment requires a pro-regenerative macrophage phenotype to be retained in vivo. Polymeric microparticles may provide a potential route to direct and sustain macrophage phenotype. Interleukin-4 (IL-4) is the most commonly used cytokine for in vitro modulation towards M2a macrophage phenotype. We designed IL-4 encapsulated microparticles to investigate the impact of drug release kinetics and developed a robust human peripheral blood monocyte cell (THP-1) in vitro assay to assess functional IL-4 release upon macrophage phenotype.

Methods

IL-4 was encapsulated with human serum album

SUBMITTER: Lee IN 

PROVIDER: S-EPMC11573512 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature