DDIT4/mTOR signaling pathway mediates cantharidin-induced hepatotoxicity and cellular damage.

ABSTRACT:

Background

Cantharidin (CTD) extracted from the traditional Chinese medicine Mylabris has significant therapeutic effects on various tumors. However, the high toxicity of CTD can cause serious liver damage, although the related molecular mechanisms remain unclear.

Methods

In this study, we established models of CTD-induced liver and L-O2 cell damage in mice in vivo and in vitro. Subsequently, liver function indicators were detected in mouse serum, while liver tissues were subjected to pathological and transmission electron microscopy observations. L-O2 cell activity was investigated using the CCK-8 assay, and the mRNA and protein expression of DNA damage-induced transcription factor 4 (DDIT4) in liver tissue and L-O2 cells was detected using qPCR, immuno

SUBMITTER: Tang W 

PROVIDER: S-EPMC11573530 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature