Iron-molybdenum cofactor synthesis by a thermophilic nitrogenase devoid of the scaffold NifEN.

ABSTRACT: The maturation and installation of the active site metal cluster (FeMo-co, Fe₇S₉CMo-R-homocitrate) in Mo-dependent nitrogenase requires the protein product of the nifB gene for production of the FeS cluster precursor (NifB-co, [Fe₈S₉C]) and the action of the maturase complex composed of the protein products from the nifE and nifN genes. However, some putative diazotrophic bacteria, like Roseiflexus sp. RS-1, lack the nifEN genes, suggesting an alternative pathway for maturation of FeMo-co that does not require NifEN. In this study, the Roseiflexus NifH, NifB, and apo-NifDK proteins produced in Escherichia coli are shown to be sufficient for FeMo-co maturation and insertion into the Ni

SUBMITTER: Paya-Tormo L 

PROVIDER: S-EPMC11573651 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature