Spatiotemporal transcriptomic map of glial cell response in a mouse model of acute brain ischemia.

ABSTRACT: The role of nonneuronal cells in the resolution of cerebral ischemia remains to be fully understood. To decode key molecular and cellular processes that occur after ischemia, we performed spatial and single-cell transcriptomic profiling of the male mouse brain during the first week of injury. Cortical gene expression was severely disrupted, defined by inflammation and cell death in the lesion core, and glial scar formation orchestrated by multiple cell types on the periphery. The glial scar was identified as a zone with intense cell-cell communication, with prominent ApoE-Trem2 signaling pathway modulating microglial activation. For each of the three major glial populations, an inflammatory-responsive state, resembling the reactive states observed in neurodegenerative contexts, was observe

SUBMITTER: Zucha D 

PROVIDER: S-EPMC11573666 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature