Size-reduced DREADD derivatives for AAV-assisted multimodal chemogenetic control of neuronal activity and behavior.

ABSTRACT: Designer receptors exclusively activated by designer drugs (DREADDs) are engineered G-protein-coupled receptors that afford reversible manipulation of neuronal activity in vivo. Here, we introduce size-reduced DREADD derivatives miniD_q and miniD_i, which inherit the basic receptor properties from the G_q-coupled excitatory receptor hM3D_q and the G_i-coupled inhibitory receptor hM4D_i, respectively, while being approximately 30% smaller in size. Taking advantage of the compact size of the receptors, we generated an adeno-associated virus (AAV) vector carrying both miniD_q and the other DREADD family receptor (κ-opioid receptor-based inhibitory DREADD [KORD]) within the maximum AAV capacity (4.7 kb), allowing us to modul

SUBMITTER: Miyake T 

PROVIDER: S-EPMC11573748 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature