Catalase-peroxidase StKatG2 from <i>Salinicola tamaricis</i>: a versatile Mn(II) oxidase that decolorizes malachite green.

ABSTRACT: Manganese (Mn) oxidation processes have garnered significant attention recently due to their potential for degrading organic pollutants. These processes are primarily catalyzed by Mn(II) oxidases. Salinicola tamaricis F01, an endophytic bacterium derived from wetland plants, has demonstrated Mn(II)-oxidizing capacity. In this study, a catalase-peroxidase, StKatG2, was cloned and overexpressed in Escherichia coli from the strain F01. The purified recombinant StKatG2 exhibited Mn(II)-oxidizing activity with K _m and K _cat values of 2.529 mmol/L and 2.82 min^-1, respectively. Optimal catalytic conditions for StKatG2 were observed at pH 7.5 and 55°C, with 45.1% activity retention after an 8-h exposure to 80°C. The biogenic manganese ox

SUBMITTER: Ding M 

PROVIDER: S-EPMC11573757 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature