Optimizing the production of recombinant human papilloma virus type 52 major capsid protein L1 in Hansenula polymorpha.

ABSTRACT: Cervical cancer is the fourth most prevalent cancer among women globally, with Thai women ranking it as the third most common. At present, a prophylactic vaccine, containing virus-like particles (VLPs) of HPV L1 capsid protein, is widely recognized as one of the major prevention strategies for cervical cancer. Unfortunately, due to a low cross-protection among subtypes, protection against each HPV subtype requires vaccination with VLPs of that specific subtype. This study aimed to optimize the cultivation medium and conditions to maximize the volumetric yield of HPV52 L1 protein produced by the recombinant H. polymorpha HPV52. The results revealed that supplementation of a complex organic nitrogen source HySoy (at 10 g/L) into SYN6 medium resulted in a significantly higher specific HPV52 L

SUBMITTER: Phimsen W 

PROVIDER: S-EPMC11574089 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature